Shannon Wallet, Ph.D.

Shannon 2Department of Oral Biology

Associate Professor

Phone: 352-273-8370
Laboratory: 352-273-8135
Room D11-23
PO Box 100424
Gainesville, FL 32610-0424


  • UNC at Chapel Hill, Chapel Hill, NC: Ph.D. – Curriculum of Oral Biology, 2000-2005
  • Duke University, Durham, NC: Clinical Laboratory Science Certification, 1994-1995
  • North Carolina State University, Raleigh, NC: Bachelor of Science – Medical Technology, 1991-1995

Previous Positions

  • 1995-2000: Medical Technologist Duke University Medical Center
    Supervisor: Mary Lee Campbell, MT (ASCP) Transfusion Services Laboratory
  • 1999-2000: Research Assistant Duke University Medical Center
    Supervisor: Nancy Reinsmoen, PhD. Immunology Transplant Laboratory
  • 2000-2005: Ph.D. Candidate University of North Carolina at Chapel Hill
    Dissertation advisor: Roland Tisch, PhD. Department of Microbiology and Immunology
  • 04/05-11/05: Post-doctoral Fellow University of Pittsburgh School of MedicinePrincipal Investigator: Chad Steele, PhD. Department of Pediatrics
  • 11/05-08/06: Scientific Consultant University of North Carolina at Chapel HillDental Research Center (DRC), Comprehensive Center for Inflammatory Disease (CCID)
  • 09/06-06/13: Assistant Professor, University of Florida, Departments of Periodontology and Oral Biology
  • 07/13-01/15: Associate Professor, University of Florida, Department of Periodontology and Oral Biology
  • 01/15-           Associate Professor, University of Florida, Department of Oral Biology

Main Interests

Shannon Wallet’s research interests are focused on mechanisms associated with altered innate immune functions, which lead to dysregulated adaptive immunity. Through her independent and collaborative research programs, she has been involved, at some level, in investigating the basic biology of health, multiple autoimmune conditions, autoinflammation, sepsis, exercise induced inflammation, and, most recently, cancer. A major focus for Wallet is understanding intestinal immune regulation under conditions of health versus disease, in the context of type 1 diabetes.  Investigations into these interactions have been hindered by a lack of robust primary cell culture techniques, which the Wallet laboratory has most recently been able to overcome.  Wallet also studies altered immunological mechanisms which lead to tissue destruction in the context of periodontal disease.  Here, through funding from the NIDCR and American Diabetes Association (ADA), Wallet has demonstrated that oral epithelial cells play a central role in regulating oral inflammation and its resolution.  In addition, she has begun to decipher osteoclast specific mechanisms associated with excessive bone destruction observed in periodontal disease of individuals with type 1 diabetes. Wallet also investigates the subversion of immunity involved in pancreatic cancer.  Through funding from the UF-sponsored Team Science Project (UF TSP) and American Cancer Society Career Development Awards (ACS CDA), Wallet has generated data allowing her to develop personalized targeting nanomedicines as well as to decipher the mechanisms associated with pancreatic cancer specific suppression of the innate immune system, allowing for the progression of disease and metastases. Dr. Wallet is keen to utilize her expertise to bring together multiple research communities with the goal of advancing human health.


  1. Seifarth C’, Pop S‘, Liu B, Wong CP, Tisch R. More stringent conditions of plasmid DNA vaccination are required to protect grafted versus endogenous islets in nonobese diabetic mice. J Immunol. 2003 Jul 1;171(1):469-76. ‘co-first authorship PMID: 12817032.
  2. Scott RS, McMahon EJ, Pop SM, Reap EA, Caricchio R, Cohen PL, Earp HS, Matsushima GK. Phagocytosis and clearance of apoptotic cells is mediated by MER. Nature. 2001 May 10;411(6834):207-11 PMID: 11346799.
  3. Pop SM, Wong CP, Culton DA, Clarke SH, Tisch R. Single cell analysis shows decreasing FoxP3 and TGFbeta1 coexpressing CD4+CD25+ regulatory T cells during autoimmune diabetes. J Exp Med. 2005 Apr 18;201(8):1333-46. PMID: 15837817
  4. Pop SM, Wong CP, He Q, Wang Y, Wallet MA, Goudy KS, Tisch R. The type and frequency of immunoregulatory CD4+ T-cells govern the efficacy of antigen-specific immunotherapy in nonobese diabetic mice. Diabetes. 2007 May;56(5):1395-402. PMID: 17317763.
  5. Maile R, Pop SM, Tisch R, Collins EJ, Cairns BA, Frelinger JA. Low-avidity CD8lo T cells induced by incomplete antigen stimulation in vivo regulate naïve higher avidity CD8hi T cell responses to the same antigen. Eur J Immunol. 2006 Feb;36(2):397-410. PubMed PMID: 16402405.
  6. Steele C, Rapaka RR, Metz A, Pop SM, Williams DL, Gordon S, Kolls JK, Brown GD. The beta-glucan receptor dectin-1 recognizes specific morphologies of Aspergillus fumigatus. PLoS Pathog. 2005 Dec;1(4):e42. Epub 2005 Dec 9 PMID: 16344862.
  7. Xiu Y, Wong CP, Bouaziz JD, Hamaguchi Y, Wang Y, Pop SM, Tisch RM, Tedder TF. B lymphocyte depletion by CD20 monoclonal antibody prevents diabetes in nonobese diabetic mice despite isotype-specific differences in Fc gamma R effector functions. J Immunol. 2008 Mar 1;180(5):2863-75.PMID: 18292508
  8. Koutouzis T, Haber D, Shaddox L, Aukhil I, Wallet SM. Autoreactivity of serum immunoglobulin to periodontal tissue components: a pilot study. J Periodontol. 2009 Apr;80(4):625-33 PMCID: PMC3594842.
  9. Tesmer M, Wallet S, Koutouzis T, Lundgren T. Bacterial colonization of the dental implant fixture-abutment interface: an in vitro study. J Periodontol. 2009 Dec;80(12):1991-7 PMID: 19961382.
  10. Mans JJ, von Lackum K, Dorsey C, Willis S, Wallet SM, Baker HV, Lamont RJ, Handfield M. The degree of microbiome complexity influences the epithelial response to infection. BMC Genomics. 2009 Aug 18;10:380. PMCID:PMC2736203.
  11. Pauley KM, Satoh M, Pauley BA, Dominguez-Gutierrez PR, Wallet SM, Holliday LS, Cha S, Reeves WH, Chan EK. Formation of GW/P bodies as marker for microRNA-mediated regulation of innate immune signaling in THP-1 cells. Immunol Cell Biol. 2010 Feb;88(2):205-12. PMCID: PMC2824770.
  12. Shaddox L, Wiedey J, Bimstein E, Magnuson I, Clare-Salzler M, Aukhil I, Wallet SM. Hyper-responsive phenotype in localized aggressive periodontitis. J Dent Res. 2010 Feb;89(2):143-8. PMCID: PMC3096871.
  13. Wallet MA, Wallet SM, Guiulfo G, Sleasman JW, Goodenow MM. IFNgamma primes macrophages for inflammatory activation by high molecular weight hyaluronan. Cell Immunol. 2010;262(2):84-8. PMCID: PMC2891046.
  14. Meka A, Bakthavatchalu V, Sathishkumar S, Lopez MC, Verma RK, Wallet SM, Bhattacharyya I, Boyce BF, Handfield M, Lamont RJ, Baker HV, Ebersole JL, Kesavalu L. Porphyromonas gingivalis infection-induced tissue and bone transcriptional profiles. Mol Oral Microbiol. 2010 Feb;25(1):61-74. PMCID: PMC3001241.
  15. Katz J, Wallet SM, Cha S. Periodontal disease and the oral-systemic connection: “Is it all the RAGE?” Quintessence Int. 2010 Mar;41(3):229-37. PMID: 20213024
  16. Aris JP*, Elios MC, Bimstein E, Wallet SM, Cha S, Lakshmyya KN, Katz J. Gingival RAGE Expression in Calorie Restricted Versus ad Libitum fed Rats. J Periodontol. 2010 Oct;81(10):1481-7 PMID: 20572766.
  17. Verma RK, Rajapakse S, Meka A, Hamrick C, Pola S, Bhattacharyya I, Nair M, Wallet SM, Aukhil I, Kesavalu L. Porphyromonas gingivalis and Treponema denticola Mixed Microbial Infection in a Rat Model of Periodontal Disease. Interdiscip Perspect Infect Dis. 2010;2010:605125. Epub 2010 May 31. PMCID: PMC2879544.
  18. Bakthavatchalu V, Meka A, Sathishkumar S, Lopez MC, Verma RK, Wallet SM, Bhattacharyya I, Boyce BF, Mans JJ, Lamont RJ, Baker HV, Ebersole JL, Kesavalu L. Molecular characterization of Treponema denticola infection-induced bone and soft tissue transcriptional profiles. Mol Oral Microbiol. 2010 Aug;25(4):260-74. PMCID: PMC3011892.
  19. Wallet SM, Chung J, Handfield M. Use of in vivo-induced antigen technology (IVIAT) to identify factors of Porphyromonas gingivalis. Methods Mol Biol. 2010;666:181-95 PMID: 20717786.
  20. Verma RK, Bhattacharyya I, Sevilla A, Lieberman I, Pola S, Nair M, Wallet SM, Aukhil I, Kesavalu L. Virulence of major periodontal pathogens and lack of humoral immune protection in a rat model of periodontal disease. Oral Dis. 2010 Oct:16 (7):686-95. PubMed PMID: 20846155.
  21. Koutouzis T, Wallet SM, Calderon N, Lundgren T. Bacterial Colonization of the Implant-Abutment Interface utilizing an in-vitro dynamic loading model. J Periodontol. 2011 Apr; 82(4):613-8. Epub 2010 Nov 2 PMID: 21043798
  22. Cuenca AG, Wynn JL, Kelly-Scumpia KM, Scumpia PO, Vila L, Delano MJ, Mathews CE, Wallet SM, Reeves WH, Behrns KE, Nacionales DC, Efron PA, Kunkel SL and Moldawer LL. Critical role for CXCL10 (IP-10)/CXCR3 signaling in the murine neonatal response to sepsis. Infect Immun. 2011 Jul:70(7):2746-54. Epub 2011 Apr 25. PMCID: PMC3191971.
  23. Delano MJ, Thayer T, Gabrilovich S, Kelly-Scumpia KM, Winfield RD, Scumpia PO, Cuenca AG, Warner E, Wallet, SM, Wallet MA, O’Malley KA, Ramphal R, Clare-Salzer M, Efron PA, Mathews CE, Moldawer LL. Sepsis Induces Early Alterations in Innate Immunity That Impact Mortality to Secondary Infection. J. Immunol. 2011 Jan1;196(1):195-202 PMCID: PMC3771366
  24. Shaddox LM, Wiedey J, Calderon NL, Magnusson I, Bimstein E, Bidwell JA, Zapert EF, Aukhil I, and Wallet SM. Local Inflammatory Markers and Systemic Endotoxin in Aggressive Periodontitis. J Dent Res. J Dent Res. 2011 Sep;90(9):1140-4. Epub 2011 Jul 5. PMCID: PMC3169885.
  25. Amir J, Watie, M, Tobler J, Catalfamo DL, Koutouzis, T and Wallet SM. The role of hyperglycemia in mechanisms of exacerbated inflammatory responses within the oral cavity. Cell Immunol. 2011;272(1):45-52. Epub 2011 Sep 29. PMCID: PMC3221011.
  26. Bickford JS, Newsom KJ, Herlihy JD, Muller C, Keeler B, Qiu X, Walters JN, Su N, Wallet SM, Flotte TR and Nick HS. Induction of Group IVC Phospholiase A2 in Allergic Ashma: Transcriptional Regualtion by TNFα in Bronchoepithelial cells. Biochem J. 2012 Feb 15;442(1) PMID: 22082005
  27. Santamaria MP, Casati MZ, Nociti Junior FH, Sallum AW, Sallum EA, Aukhil I, Wallet SM, and Shaddox LM. Connective tissue graft plus resin-modified glass ionomer restoration for the treatment of gingival recession associated with non-carious cervical lesions: microbiological and immunological results. Clinical Oral Investig. 2012 Feb 29 [Epub ahead of print] PMID: 22373777.
  28. Koutouzis T, Catania D, Neiva K, Wallet SM. Innate Immune Receptor Expression in Perio-implant tissues of patients with different susceptibility to periodontal diseases. J Periodontol. Epub 2012 Apr 2. PMID: 22468681.
  29. Toro EJ, Zuo J, Ostrov DA, Catalfamo DL, Bradaschia-Correa V, Arana-Chavez V, Caridad AR, Neubert JK, Wronski TJ, Wallet SM, and Holiday LS. Enoxacin directly inhibits oseoclastogenesis without inducing apoptosis. J Biol Chem. 2012 May 18;287(21):17894-904. Epub 2012 Apr 2 PMCID: PMC3366803.
  30. Koutouzis T, Mesia R, Calderon N, Wong F, Wallet S. The Effect of Dynamic Loading on Bacterial Colonization of the Dental Implant Fixture-Abutment Interface: An in-vitro study. J Oral Implatol. 2012 May 7. Epub ahead of print. PMID: 22564184.
  31. Welc SS, Phillips NA, Oca-Cossio J, Wallet SM, Chen DL, and Clanton TL. Hyperthermia increases interleukin-6 in mouse skeletal muscle. Am J Physiol Cell Physiol. 2012 Aug 15:303(4);C455-66 Epub 2012 Jun 6. PMCID: PMC3422986
  32. Catalfamo DL, Calderon NL, Harden S, Neiva KG, and Wallet SM. Augmented LPS Responsiveness in Type 1 Diabetes-Derived Osteoclasts. J Cell Physiol. 2013 Feb;228(2):349-61 PMCID: PMC3470828.
  33. Beliveau D, Magnusson I, Bidwell JA, Zapert EF, Aukhil I, and SM Wallet, and Shaddox LM. Benefits of Early Systemic Antibiotics in Localized Aggressive Periodontitis: A Retrospective Study. Journal of Clinical Periodontology. 2012 Nov;39(11):1075-81. Epub 2012 Aug 29. PMCID: PMC3467316.
  34. Cruz-Almeida Y, King CD, Wallet SM, Riley JL 3rd. Immune biomarker response depends on choice of experimental pain stimulus in healthy adults: a preliminary study. Pain Res Treat. 2012:2012:538739 Epub 2012 Nov 20. PMCID: PMC3508574.
  35. Wallet MA, Calderon NL, Alonso TR, Choe CS, Catalfamo DL, Lalane CJ, Neiva KG^, Panagako F and Wallet SM. Triclosan Alters Anti-microbial and Inflammatory Responses of Epithelial Cells. Oral Diseases. 2013 Apr;19(3):296-302. PMCID: PMC3800022.
  36. Catalfamo DL, Britten TM, Storch DI, Calderon NL, Sorenson HL, and Wallet SM. Hyperglycmia Induced and Intrinsic Alterations in Type 2 Diabetes-Derived Osteoclast Function. Oral Diseases. 2013 Apr;19(3):303-12. PMCID: PMC3800028.
  37. Rodrgues PH, Reyes L, Chadda AS, Belanger M, Wallet SM , Akin D, Dunn W Jr, Progulske-Fox A. Porphyromonas gingivalis Strain Specific Interactions with Human Coronary Artery Endothelial Cells: A Comparative Study. PLoS One. 2012; 7(12):e52606 Epub 2012 Dec 26. PMID: 23300720.
  38. Knowlton AE, Fowler LJ, Patel RK, Wallet SM, and Grishaber SS. Chalmydia Induces Anchorage Independence in 3T3 Cells and Detrimental Cytological Defects in an Infection Model. PLoS One. 2013;8(1):e54022 Epub 2013 Jan 7. PMCID: PMC3538680.
  39. Shaddox LM, Goncalves PF, Vovk A, Allin N, Huang H, Hou W, Aukhil I, Wallet SM. LPS-induced inflammatory response after therapy of aggressive periodontitis. J Dent Res. 2013 Aug:92(8):702-8. Epub 2013 Jun 20. PMCID: PMC3711573.
  40. Reyes L, Eiler-McManis E, Rodrigues PH, Chadda AS, Wallet SM, Bélanger M, Barrett AG, Alvarez S, Akin D, Dunn WA Jr, Progulske-Fox A. Deletion of lipoprotein PG0717 in Porphyromonas gingivalis W83 reduces gingipain activity and alters trafficking in and response by host cells. PLoS One. 2013 Sep 12;8(9):e74230 PMCID: PMC3772042.
  41. Neiva KG, Calderon NL, Alonso TR, Panagakos F, Wallet SM. Type 1 diabetes-associated TLR responsiveness of oral epithelial cells. J Dent Res. 2014 Feb;93(2):169-74. Epub 2013 Dec 13. PubMed PMID:24334435.
  42. Gonçalves PF, Klepac-Ceraj V, Huang H, Paster BJ, Aukhil I, Wallet SM, Shaddox LM. Correlation of Aggregatibacter actinomycetemcomitans detection with clinical /immunoinflammatory profile of localized aggressive periodontitis using a 16S rRNA microarray method: a cross-sectional study. PLoS One. 2013 Dec 23;8(12) PMCID: MC3871691.
  43. Neiva KG, Catalfamo DL, Holliday S, Wallet SM, Pileggi R. Propolis decreases lipopolysaccharide-induced inflammatory mediators in pulp cells and osteoclasts. Dent Traumatol. 2014 Feb 6. [Epub ahead of print] PubMed PMID: 24502251.
  44. Park E, Na HS, Kim SM, Wallet S, Cha S, Chung J. Xylitol, an Anti-caries Agent, Exhibits Potent Inhibition of Inflammatory Responses in Human THP-1-derived Macrophages Infected with Porphyromonas Gingivalis. J Periodontol. 2014 Mar 4. [Epub ahead of print] PubMed PMID: 24592909.
  45. Rody WJ Jr, Holliday LS, McHugh KP, Wallet SM, Spicer V, Krokhin O. Mass spectrometry analysis of gingival crevicular fluid in the presence of external root resorption. Am J Orthod Dentofacial Orthop. 2014 Jun;145(6):787-98. PMID: 24880850
  46. Koutouzis T, Mesia R, Calderon N, Wong F, Wallet S. The effect of dynamic loading on bacterial colonization of the dental implant fixture –abutment interface: an in vitro study. J Oral Implantol. 2014 Aug: 40(4):432-7. PMID: 25106007/
  47. Graves CL, Harden SW, LaPato M, Nelson M, Amador B, Sorenson H, Frazier CJ, Wallet SM. A method for high purity intestinal epithelial cell culture from adult human and murine tissues for the investigation of innate immune function. J Immunol Methods. 2014 Dec;414:20-31. Epub 2014 Sep 2. PMID: 25193428
  48. Jones MK, Watanabe M, Zhu S, Graves CL, Keyes LR, Grau KR, Gonzalez-Hernandez MB, Iovine NM, Wobus CE, Vinjé J, Tibbetts SA, Wallet SM, Karst SM. Enteric bacteria promote human and mouse norovirus infection of B cells. Science. 2014 Nov 7;346(6210):755-9. PMID: 25378626
  49. Lu S, Zeumer L, Sorensen H, Yang H, Ng Y, Yu F, Riva A, Croker B, Wallet S, Morel L. The Murine Pbx1-d Lupus Susceptibility Allele Accelerates Mesenchymal Stem Cell Differentiation and Impairs Their Immunosuppressive Function. J Immunol. 2014 Nov 21 [Epub ahead of print] PMID: 25416808
  50. Phillips NA, Welc SS, Wallet SM, King MA, Clanton TL. Protection of intestinal injury during heat stroke in mice by interleukin-6 pretreatment. J Physiol. 2014 Nov 28. [Epub ahead of print] PMID:25433073
  51. Cuenca AG, Joiner DN, Gentile LF, Cuenca AL, Wynn JL, Kelly-Scumpia KM, Scumpia PO, Behrns KE, Efron PA, Nacionales D, Lui C, Wallet SM, Reeves WH, Mathews CE, Moldawer LL.TRIF-Dependent Innate Immune Activation Is Critical for Survival to Neonatal Gram-Negative Sepsis. J Immunol. 2014 Dec 29. [Epub ahead of print] PMID: 25548220
  52. Delitto D, Pham K, Vlada AC, Sarosi GA, Thomas RM, Behrns KE, Liu C, Hughes SJ, Wallet SM, Trevino JG. Patient-Derived Xenograft Models for Pancreatic Adenocarcinoma Demonstrate Retention of Tumor Morphology through Incorporation of Murine Stromal Elements. Am J Pathol. 2015 Mar 12.. [Epub ahead of print] PMID: 25770474